Figure 5. The learning deficit is not caused by ethanol anesthesia.

The ethanol treatment did not reduce the sensitivity of larvae to the heat treatment (US). Larvae were placed onto an agar dish in which half of the dish is 24°C and the other half is held at a different temperature. An avoidance index was calculated based on how many larvae avoided the artificially heated or cooled half of the plate. Shown are plots indicating the avoidance index at every minute for the total duration of heat-avoidance assay. The dotted lines with filled squares are ethanol treated group and solid lines with empty circles are the water treated group. The ethanol and water treated groups are not different for any temperature tested (p>0.05 for all points).