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. 2012 May 18;7(5):e37272. doi: 10.1371/journal.pone.0037272

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Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The indicated cell lines were treated with sodium butyrate for 48 h to induce erythroid differentiation. The cells were then incubated with FITC-conjugated BS-I Isolectin B4 from Bandeiraea simplicifolia. Flow cytometric analyses were then performed to determine the levels of surface B antigen expression. Representative histograms for the indicated sublines were shown. The FITC-derived fluorescent intensity was displayed on the x-axis on a logarithmic scale and the number of cells on the y-axis.