Figure 4. Expression of B3 cDNA is sufficient to induce typical mixed-field agglutination.

A. The indicated K562 sublines were treated with sodium butyrate to induce erythroid differentiation. The cells were then incubated with anti-B antibody and cell agglutination was observed using a phase contrast microscope. B. The numbers of aggregation with the indicated number of cells/aggregate were counted. Histogram for the agglutination size distribution was shown. The data represent the mean ± S.D. of three independent experiments. P<0.001 by Kruskal-Wallis test.