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. 2012 May 18;7(5):e37310. doi: 10.1371/journal.pone.0037310

Figure 1. Physical maps for pMW1650 and pRIE.

Figure 1

Both pMW1650 [9] and pRIE contain a Himar1 transposase gene under the control of the Borrelia burgdorferi flgB promoter, and a kanamycin resistance gene (KanR). Within the transposable element, bounded by inverted repeats (IR), are an E. coli ColE1 origin of replication, a GFPUV gene under the control of the R. rickettsii ompA promoter, and a rifampicin resistance gene (RifR) under the control of the rpsL promoter. In addition, pRIE contains two multiple cloning sites: MCS1 for insertion of fluorescent protein genes under the control of the R. rickettsii ompA promoter, and MCS2 for insertion of other genes to be expressed under the control of their native promoter.