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. 2012 May 18;7(5):e37654. doi: 10.1371/journal.pone.0037654

Figure 1. The purification of MoHrip1 from M. oryzae.

Figure 1

A. Concentrated culture filtrate was loaded on HiTrap™ Q HP 5 ml column at a flow rate of 2 ml/min. Four peaks were collected, and the target protein was included in peak a2. B. Peak a2 was pooled and applied to native-PAGE and electroelution. The b3 fraction was the target elicitor protein. C. SDS-PAGE analysis of the purified elicitor protein, MoHrip1, showing a single band with Coomassie Brilliant Blue R-250 staining. (1: MoHrip1, M: protein molecular weight marker).