Figure 5. MoHrip1-induced callose deposition and extracellular medium alkalinization in tobacco.

A. Callose deposition in tobacco leaves. Tobacco leaves were infiltrated with MoHrip1 (50 µL of a 5 µM solution of MoHrip1) or Mes-NaOH buffer (20 mM, pH 6.0) as a control. After a 24 hour incubation period, the tobacco leaves were bleached to remove their chlorophyll and then stained with aniline blue. The samples were observed under bright-field (a and c) and UV fluorescence (b and d) microscopy. Apparent punctiform callose deposits around the cell wall were photographed in the MoHrip1-treated leaves (b). a and b: MoHrip1-treated leaves, c and d: control. Scale bar = 10 µM. B. The kinetics of the extracellular medium alkalinization induced by MoHrip1 in tobacco suspension. A distinct pH increase in the elicitor-treated cell culture was monitored for the first 10 minutes, and the pH stabilized after 100 minutes. Each data point represents three replicates. The error bars represent ± SD of the mean.