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. 2012 May 18;7(5):e37654. doi: 10.1371/journal.pone.0037654

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Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A H₂O₂ burst was obviously elicited by MoHrip1 (dissolved in sterile distilled water) or flg22 (positive control, dissolved in sterile distilled water) within 10 minutes in rice leaves, but no H₂O₂ burst was detected in the samples treated with sterile distilled water (negative control). After 20 minutes, the level of ROS production in MoHrip1- or flg22-treated samples decreased to that in the negative control samples. The error bars represent ± SD of the mean. Essentially identical results were obtained in three independent experiments.