Figure 6. Free energy profiles of methyl transfer processes in the Y1124F mutant.

The first methyl transfer: blue and solid line with a free energy barrier of 13.9 kcal/mol; the second methyl transfer: red and dashed line with a free energy barrier of 15.9 kcal/mol; the third methyl transfer: green and dashed line with a free energy barrier of 13.3 kcal/mol.