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. 2012 May 18;7(5):e37592. doi: 10.1371/journal.pone.0037592

Figure 1. CtsKCreERT2 construct and In vitro functionality.

Figure 1

A: Schematic diagram of transgenic CtsKCreERT2 expression construct used to generate transgenic mice, which includes the full sequence of the CtsK promoter, the CreERT2 fusion sequence, and the SV40 polyA signal. B. Schematic representation of the floxed neomycin construct before and after Cre-mediated recombination and expected size of PCR products. C: Cre-mediated recombination in Hela cells (upper panel). Hela cells transfected with the floxed neomycin plasmid only (lane 1) or co-transfected with CtsKCreERT2 and floxed nemycin constructs and treated (lane 2) or not (lane 3) with 4-OHT. Untrasfected Hela cells are shown in lane 4. The recombination resulted in a 200 bp PCR fragment. Cre expression was also monitored in the same samples by PCR (lower panel). M: molecular weight markers.