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. 2012 May 18;7(5):e37592. doi: 10.1371/journal.pone.0037592

Figure 4. Cre-mediated β-galactosidase expression in tissue sections of transgenic embryos.

Figure 4

E17.5 embryos obtained after crossing CtsKCreERT2 positive or wild-type females with ROSA26 males were processed and stained for β-galactosidase activity as described under materials and methods. A: E17.5 C57BL/6 wild-type control embryos; a.1: overview of β-gal activity; a.2, a.3 and a.6: arms; a.4 and a.5: legs; a.7: spinal cord B: E17.5 CtsKCreERT2+/−LacZ+/+ embryos (strain #4); b.1: overview of β-gal activity. b.2 and b.3: arms; b.4 and b.5 legs; b.6: spinal cord. Arrows show positive β-galactosidase activity in bones. C: E17.5 CtsKCreERT2−/−LacZ+/+ embryos (strain #4); c.1: overview of β-gal activity; c.2 and c.3: arms; c.4 and c.5: legs; c.6: spinal cord.