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. 2012 May 18;7(5):e35369. doi: 10.1371/journal.pone.0035369

Table 1. Summary statistics of Scophthalmus maximus 454-pyrosequencing.

Data generation and filtering
Number of total reads 915,782
Total Megabases 291.04
Average read length (bp) 317.8
N50 read length (bp) 396
Number of high quality reads (after filtering) 915,256 (99.94%)
Total Megabases (after filtering) 271.95
Average read length (after filtering) 297.14
N50 read length (after filtering) 381
Assembly statistics
Number of reads assembled 733,411 (80.13%)
Number of contigs 55,404
Total consensus Megabases 37.2
Average contig coverage 4.4
Average contig length 671.3
N50 contig length 756
Range contig length 40–6,821
Number of contigs >500 pb 31,764 (57.33%)
Number of contigs with 2 reads 21,270 (38.39%)
Number of contigs with >2 reads 34,134 (61.61%)
Number of clusters 41,870
Number of clusters with 1 contig 39,578 (94.53%)
Number of clusters with >1 contig 2,292 (5.47%)
Number of singletons 181,845
Annotation
Total number of annotated contigs 24,845 (44.84%)
SwissProt+Metazoan RefSeq hits 20,175 (81.2%)
nr hits 4,670 (18.8%)
Unique protein hits 12,591
Total number of annotated singletons 13,722