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. 2012 May 18;7(5):e36928. doi: 10.1371/journal.pone.0036928

Table 1. Sequences of primer used for real-time PCR assay.

Target sequence Sequence (5′-3′) size (bp)
β-actin R: AGAGGGAAATCGTGCGTGACF: CAATAGTGATGACCTGGCCGT 139
RAE-1 R: ATCAACTTCCCCGCTTCCAF: AGATATGAAGATGAGTCCCACAGAGATA 79
H60 R: GAGCCACCAGCAAGAGCAAF: CCAGTATGGTCCCCAGATAGCT 75
MULT-1 R: TTCACATAGTGCAGGAGACTAACACAF: ACTGGCCACACACCTCAGC 69
Qa-1a R: GCGGTATTTCCACACTGCCAF: TCTGTGAGGCAAAGTCAGTC 80
Qa-1b R: CCTGGACCGCGAATGACATAF: CACCACAGCTCCAAGGATGAT 514
NKG2D R: ACGTTTCAGCCAGTATTGTGCF: GGAAGCTTGGCTCTGGTTC 132
TRAIL R: CCCTGCTTGCAGGTTAAGAGF: G GCCTAAGGTCTTTCCATCC 240
FasL R: AAGAAGGACCACAACACAAATCTGF: CCCTGTTAAATGGGCCACACT 233
Perforin R: GAGAAGACCTATCAGGACCAF: AGCCTGTGGTAAGCATG 166
TLR3 R: TCTGGAAACGCGCAAACCF: GCCGTTGGACTCTAAATTCAAGAT 85
TLR4 R: AGAAATTCCTGCAGTGGGTCAF: TCTCTACAGGTGTTGCACATGTCA 84
IL-18 R: GCCATGTCAGAAGACTCTTGCGTCF: GTACAGTGAAGTCGGCCAAAGTTGTC 122
IL-15 R: TTAACTGAGGCTGGCATTCATGTCTTCF: CAGTTCATTGCAGTAACTTTGCAACTG 194
IL-12p35 R:CATCGATGAGCTGATGCAGTF: CAGATAGCCCATCACCCTGT 163
IL-12p40 R:TGGAAGCACGGCAGCAGAATAAATF:TGCGCTGGATTCGAACAAAGAACT 202
IFN-β R:CCATCATGAACAACAGGTGGATF:GAGAGGGCTGTGGTGGAGAA 67
IFN-γ R: TGCATCTTGGCTTTGCAGCTCTTCCTCATGGCF: TGGACCTGTGGGTTGTTGACCTCAAACTTGGC 194
IFN-α R: TCTGTGCTTTCCTCGTGATGF: TTGAGCCTTCTGGATCTGCT 215