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. 2012 May 18;7(5):e36142. doi: 10.1371/journal.pone.0036142

Figure 5. MSS1 gene silencing inhibited microglial NF-kB activation in response to LPS stimulation.

Figure 5

The BV2 cells were transfected with either MSS1-specfic or control siRNA for 24 h, then cells were incubated with LPS at 1000 ng/mL for 8 h. After that, cells were transfected with NF-kB-luciferase reporter plasmid and pCMV-gal control vectors using Lipofectamine reagents. NF-kB activation was detected and expressed as relative luciferase activity. Compared with the negative control group, treatment with MSS1-targetd siRNA significantly suppressed the enhancement of NF-kB activity by LPS. Data were obtained from three independent experiments with four to six replicates each. *p<0.05 compared with the negative control group.