Figure 3. Cytokine production by T lymphocytes from the vaccinated macaques.

CD8⁺ cells (A, B) and CD4⁺ cells (C, D) were separated from the blood cells 2 weeks after the second vaccination (week 4). T lymphocytes were cultured with irradiated APC and the whole particle or split vaccine antigen (10 µg/ml) for 48 h. Culture without antigen is indicated as medium. Cytokines (IFN-γ; A, C, IL-2; B, D) in the supernatants were measured with a multiple cytokine array. IL-4 production was under the detection limit.