Figure 7. NP peptides bound to Mafa-A1*052∶02.

(A) Amino acid sequences of 10-mer peptides derived from Narita1 nucleoprotein are shown as one-letter code. The sequence of influenza A virus PB1 peptide was used as a representative of non-binding peptides to Mafa-A1*052∶02. The numbers in parentheses indicate residues from the N-terminus of NP. (B) RMA-S expressing Mafa-A1*052∶02 and human β2-microglobulin was cultured with NP peptides at 27°C for 1 h and then at 37°C for 4 h. Stable expression of Mafa-A1*052∶02 on the cell surface was examined by staining with W6/32 antibody. Orange lines: cells cultured with indicated peptides. Blue shade: cells cultured without peptide. (C) CD8⁺ cells were isolated from spleens of indicated macaques and cultured with APC and indicated peptides. IFN-γ production was determined using ELISA. Averages and SD of triplicate culture are shown. Representative results of three independent experiments are shown.