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. 2012 May 18;7(5):e37039. doi: 10.1371/journal.pone.0037039

Figure 5. Let-7a interacts with the 3′-untranslated region (UTR) of integrin β3.

Figure 5

(a) The potential let-7a binding site in mouse integrin-β3 (Itgb3). (b) Luciferase activities of the wild type or mutated integrin-β3 3′UTR reporter constructs co-electroporated with the let-7a precursor in HeLa cells. Dual luciferase assays were performed in cell lysates at 48-hour post-electroporation. (c) Western blot analysis of integrin-β3 protein from blastocysts electroporated with pre-let7a or control RNA at the 8-cell embryo stage. Each lane contained extract from 40 blastocysts. Semi-quantitative analysis of integrin-β3 expression levels in the embryos. There was a significant decrease in integrin-β3 protein expression in embryos eclectroporated with pre-let-7a relative to the control. The data represent the mean and standard errors (n = 3). *denotes P<0.05.