Table 4. Expression of let-7 family members (in Ct values) in dormant and activated blastocysts.
MiRNA | Dormant (Dor) | Activated (Act) | fold change Act/Dor | ||||||
1 | 2 | 3 | Mean | 1 | 2 | 3 | Mean | ||
mmu-let-7b | 18.5 | 18.0 | 18.4 | 18.3 | 18.6 | 18.8 | 18.9 | 18.8 | 0.72 |
mmu-let-7c | BD | BD | BD | − | BD | BD | BD | − | − |
mmu-let-7d | 26.7 | 26.3 | 26.5 | 26.5 | BD | BD | BD | − | ∞ |
mmu-let-7e | 20.7 | 20.8 | 20.9 | 20.8 | 24.7 | 24.5 | 24.7 | 24.6 | 0.07 |
mmu-let-7i | BD | BD | BD | − | BD | BD | BD | − | − |
mmu-let-7g | 21.6 | 21.9 | 21.6 | 21.7 | 23.6 | 23.7 | 23.9 | 23.7 | 0.25 |
mmu-let-7a | 21.2 | 21.4 | 21.0 | 21.2 | 26.2 | 26.6 | 26.5 | 26.4 | 0.03 |
mmu-let-7d* | 18.9 | 18.5 | 18.2 | 18.5 | 19.0 | 18.9 | 18.6 | 18.8 | 0.80 |
mmu-let-7f | 19.0 | 19.5 | 19.2 | 19.2 | 24.6 | 23.9 | 24.0 | 24.2 | 0.03 |
BD = below detection limit.