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. 2012 May 18;7(5):e37107. doi: 10.1371/journal.pone.0037107

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Shimma et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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This product ion spectrum is an enlarged view of the m/z 560–880 region in Figure 4. From the peak pattern and the mass value of the precursor ion, m/z 888 was postulated as ST(d18∶1, C24∶1). The regular peak patterns obtained via the CRF process were largely assigned as product ions from a fatty acid moiety (C24∶1) and a long-chain base (d18∶1). Furthermore, the carbon–carbon double bond at the n−9 position was confirmed from the CRF sequencing pattern.