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. 2012 May 18;7(5):e37136. doi: 10.1371/journal.pone.0037136

Figure 3. Evaluation of the CWTrAP system using α-factor peptide for yeast endogenous Ste2 receptor.

Figure 3

(A) Pheromone signaling assays of α-factor-displaying yeast strains. Error bars represent the standard deviation of three independent experiments. (B) Immunofluorescence staining of α-factor displaying yeast strains. Anti-Flag antibody and Alexa Fluor 546-conjugated secondary antibody were used for detection of secreted α-factor or α-factor-anchor fusion proteins. IMG-4 was used as the host strain. The transformants used in these experiments are listed in Table S3. Sec: free, secreted form of α-factor. AG: C-terminal half of α-agglutinin anchor.