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Immunofluorescence staining of somatostatin displaying yeast strains. Anti-Flag antibody and Alexa Fluor 594-conjugating secondary antibody were used for detection of Flag–Flo42 anchor or S-14–Flag–Flo42 fusion proteins. Red fluorescence images are shown in false-color. IMFD-70 was used as the host strain. The transformants used in these experiments are listed in Table S3.
