Figure 4.

HoxD10 induces cell apoptosis and cell-cycle inhibition. (A) The rate of cell apoptosis after stably transfected with HoxD10 was determined by flow cytometry in AGS and MKN28 cell lines. Region A1 indicates early apoptotic cells, A2 shows late apoptotic cells. Experiments were performed in duplicate. (B) Cell-cycle distribution was analyzed by FACS flow cytometry in AGS and MKN28 cells stably transfected with pcDNA3.1-HoxD10 or pcDNA3.1 vector. The mean percentages of cell-cycle phases from representative experiments are shown in each panel.