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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Biomaterials. 2012 Apr 22;33(21):5325–5332. doi: 10.1016/j.biomaterials.2012.04.004

Fig. 5.

Fig. 5

Time-lapse phase contrast images of HUVECs under fluidic condition by a wide-field microscope (objective 10x). Perfusion started with gentle flow (2μl/min). Cells were round at this point. Flow rate was gradually increased until it reached 20ml/min. After 7 hours of perfusion, flow rate was increased to 200μl/min and HUVECs began to spread out. After 12 hours of perfusion, flow rate was increased to 2ml/min and maintained thereafter.