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. Author manuscript; available in PMC: 2013 May 18.
Published in final edited form as: ACS Chem Biol. 2012 Mar 8;7(5):911–919. doi: 10.1021/cb200349v

Figure 8.

Figure 8

Dopamine receptor expression increases cilium length in fibroblasts (a–e) Flag-tagged transferrin receptor expressed as a control. (f–j) Flag-tagged dopamine D1 receptor expression. (a,f) Untransfected control cells. Receptor in green, acetylated tubulin labeling cilia in red, DAPI labeling nuclei in blue. Scale bars are 10μm. (b) Transferrin receptors are not localized to cilia. (g) D1 receptors are localized to cilia (c,h) Acetylated tubulin labels cilia brightly. (d,i) DAPI labeling of cell nuclei. (e,j) Merged images show increased cilium length with D1 receptor expression compared to transferrin receptor controls. (k) Quantification of cilium length showing significant increase in cilium length with D1 receptor expression compared to untransfected controls on same coverslip (N=28 for D1 receptor expression and for corresponding untransfected controls, p<0.0001). The transferrin receptor control showed a slight but statistically insignificant reduction in length compared to untransfected controls (N=31 for transferrin expression, N=29 for corresponding untransfected control, p=0.0748). Error bars are 95% confidence intervals.