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. Author manuscript; available in PMC: 2013 Jun 1.

Published in final edited form as: Peptides. 2012 Mar 30;35(2):202–211. doi: 10.1016/j.peptides.2012.03.020

(A) Stomach and colon tissue sections were stained with anti-rat CLR (RK11) or RAMP1 (9891) antibodies (left panels) or with preadsorbed antibodies (right panels). CLR-IR was detected in myenteric neurons and nerve bundles throughout the circular muscle and longitudinal in the stomach. CLR- and RAMP1-IR was observed in myenteric neurons of the colon. Preadsorption of primary antibodies abolished signals for both CLR and RAMP1 indicating specificity of detection. (B). Submucosal neurons were also stained in the ileum and colon, and CLR immunoreactive fibers were more prominent in the myenteric plexus. Myn, myenteric neurons, cm, circular muscle, nf, nerve fiber, sbm, submucosal neurons. Scale bar = 33 μm.

(A) Stomach and colon tissue sections were stained with anti-rat CLR (RK11) or RAMP1 (9891) antibodies (left panels) or with preadsorbed antibodies (right panels). CLR-IR was detected in myenteric neurons and nerve bundles throughout the circular muscle and longitudinal in the stomach. CLR- and RAMP1-IR was observed in myenteric neurons of the colon. Preadsorption of primary antibodies abolished signals for both CLR and RAMP1 indicating specificity of detection. (B). Submucosal neurons were also stained in the ileum and colon, and CLR immunoreactive fibers were more prominent in the myenteric plexus. Myn, myenteric neurons, cm, circular muscle, nf, nerve fiber, sbm, submucosal neurons. Scale bar = 33 μm.