Lung tissue homogenate levels of pro-inflammatory cytokine, IL-6 (A), and neutrophil chemotactic proteins, MIP-2 (D), were significantly decreased in lungs from Hem/CLP TNF−/− mice when compared with Hem/CLP background, TNF+/+ mice. Neutrophil chemotactic protein, KC, was significantly decreased in lung tissue (C) and IL-10 (F) showed significant decreased in plasma. (n=7–8. *p<0.05 vs. equivalent Sham, @<p0.05vs TNF−/− Hem/CLP).