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. Author manuscript; available in PMC: 2013 May 18.
Published in final edited form as: ACS Chem Biol. 2012 Feb 24;7(5):871–878. doi: 10.1021/cb200497q

Figure 3.

Figure 3

Autoradiograms show DEPC probing of H69 in wild-type 50S subunits and 70S ribosomes under low and high Mg2+ conditions with longer (a) and shorter (b) gel exposure times. Chemical modification sites are shown in red on the right side of the gel. (c) Quantification analysis for the DEPC probing is shown (inset: normalized band intensity at position 1919). (d) The proposed A1913 conformational change is illustrated. Autoradiograms for SHAPE (e) and DMS (f) analyses under 6 and 20 mM Mg2+ concentrations are shown.