Table 1. Distinct cytokine release profiles by HSPs.
RAW264.7 cells were pulsed with gp96, calreticulin or HSP70 or PBS for 20 hr. Supernatants were harvested and analyzed with a mouse cytokine array kit and quantified with Kodak imager.
| gp96 | Calreticulin | Hsp70 | Regulation | |
|---|---|---|---|---|
| IL-1ra | ++++ | ++ | − | NF-κB |
| G-CSF | + | + | − | MAPK |
| IL-6 | +++ | +++ | + | NF-κB/MAPK |
| CXCL10 | + | + | + | NF-κB |
| CCL17 | + | + | + | NF-κB |
| IL-12p70 | ++ | ++ | ++ | NF-κB |
| CXCL11 | − | + | − | NF-κB |
| TREM1 | ++++ | ++ | − | unknown |
| IL-1β | +++ | +++ | +++ | NF-κB |
| TNF-α | + | ++++ | − | NF-κB |
+ indicates fold increase over PBS pulsed cells. Data is representative of 3 independent experiments.