FIGURE 2.
Assessment of Th9 cell plasticity. Th9 cells collected following 3 days of activation/polarization were expanded with IL-2 for 4 days, washed, and reactivated for 3 days with HEL and APC in the presence of polarizing cytokines specific for Th1, Th2, Th17, and Th9. Intracellular expression of IL-10, IFN-γ, IL-17, IL-4, and IL-9. A representative experiment; similar data were obtained in another experiment. This figure is modified from Ref. 33, with permission from the Journal of Immunology.