Fig. 1.
TABFOs have an Mr of ∼80,000 (∼19 Abeta42 monomers), with morphology distinct from the morphology of Abeta42 fibers. (A) TABFOs have an Mr of 60,000 ± 4,200 by SEC. Two SEC runs are superposed. Dark trace, TABFOs; light trace, protein calibration standards. The peaks of the protein standards are labeled with the corresponding molecular masses. A graphical analysis from these runs is shown in Fig. S1B. (B) TABFOs have an Mr of 100,000 ± 3,500 by TEM. Shown is the distribution of measured Mr for a randomly selected sample of 50 TABFO particles from the TEM image in C. The mean of the SEC and TEM Mr measurements is 80,000 ± 20,000. (C) TABFOs imaged by TEM. (Scale bar: 100 nm.) (D) Fibers from TABFOs grown overnight at 37 °C in PBS. (Scale bar: 200 nm.)