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. 2012 Apr 17;109(20):E1258–E1266. doi: 10.1073/pnas.1205150109

Fig. 2.

Fig. 2.

OTII T cells form motile, stable conjugate pairs with either LPS or CpG mOVA B cells. Imaging was performed in the B-cell follicle 18 h after T-cell adoptive transfer. (A) Snapshots taken during an imaging record (Movie S1) of an OTII T-cell (T, blue) and LPS mOVA B-cell (B, green) conjugate pair 18 h after adoptive transfer of OTII T cells (imaging time indicated in min:s). White arrows indicate the direction of conjugate pair movement; no arrow indicates stoppage. (Scale bar, 5 μm.) (B) Snapshots from Movie S2 of an OTII T-cell (T, blue) and CpG mOVA B-cell (B, magenta) pair 18 h after adoptive transfer of OTII T cells. (Scale bar, 5 μm.) (C) (Upper) 3D plots of LPS mOVA B–T pairs (B cell in green, T cell in blue; n = 5 pairs), normalized to the point of initial contact. (Lower) Corresponding tracks of CpG mOVA B–T pairs (B cell in red, T cell in blue; n = 5 pairs). (D) 3D coordinates (x,y,z) of OTII T cells in conjugate pairs with LPS mOVA B cells (Upper) or CpG mOVA B cells (Lower). (E) Distributions of instantaneous velocities of unpaired T cells in the presence of LPS B cells (light gray bars, mean = 10.0 ± 0.22 μm/min, n = 517 cells) or CpG B cells (dark gray bars, 10.9 ± 0.26 μm/min, n = 304); and of paired T cells while in contact with LPS B cells (green bars, 4.4 ± 0.1 μm/min, n = 843) or CpG B cells (red bars, 5.4 ± 0.1 μm/min, n = 1083). (F) Scatter plots showing individual and mean (red horizontal lines) durations of contacts of OTII T cells with LPS mOVA B cells (mean = 20.1 ± 2.9 min, n = 69 contacts) and CpG mOVA B cells (mean = 20.8 ± 2.4 min, n = 69 contacts) in four separate experiments for each condition. Filled circles represent B–T contacts for which the initiation or termination of contact was not visualized. (G) Contact maps of interactions between OTII T cells and LPS or CpG mOVA B cells. Lengths of colored lines indicate duration of contacts between T cells and LPS mOVA B cells (green) or CpG mOVA B cells (red); times when T cells were not in contact are indicated by gray. Data are ordered from top to bottom by total duration for which T cells were tracked.