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. 2012 Apr 17;109(20):E1258–E1266. doi: 10.1073/pnas.1205150109

Fig. 3.

Fig. 3.

Naive mOVA B-cell–OTII T-cell interactions and antigen specificity of conjugate pairs. Lengths of colored lines in contact maps (A, C, E) indicate duration of contacts between T cells as indicated on y axis and B cells indicated in label. (A) Contact map of OTII T-cell interaction history 18 h after adoptive transfer. (B) Duration of contact of OTII T cells with naive WT B cells averaged 1.1 ± 0.2 min. Duration of contact with naive mOVA B cells averaged 1.4 ± 0.1 min. n = 53 contacts each in three separate experiments. (C) Contact map of OTII T-cell interactions with naive mOVA B cells 72 h after adoptive transfer of T cells. (D) The mean duration of contact between OTII T cells and naive mOVA B cells was 8.3 ± 2.1 min for n = 54 contacts in three separate experiments. (E) Contact map of interactions between WT T-cell and WT B cells activated with LPS or CpG. (F) Mean contact durations = 2.2 ± 0.2 and 2.7 ± 0.6 min, and n = 60 and 99 contacts, respectively, in three separate experiments. Filled circles represent B–T contacts for which the initiation or termination of contact was not visualized.