Fig. 5.

Predominance of LPS mOVA B-cell–OTII T-cell conjugate pairs in the presence of cotransferred CpG mOVA B cells. (A) Interactions between CD4⁺ OTII T cells (blue) and either LPS mOVA B cells (green, green tracks) or CpG mOVA B cells (red, red tracks). Tracks of conjugate pairs are indicated in the right panel (Movie S6). (B) Close-up snapshots of an LPS mOVA B-cell (B, green)–OTII T-cell (T, blue) conjugate pair (Upper) and a brief interaction between a CpG mOVA B cell (B, red) and an OTII T cell (T, blue) (Lower). (C) 3D tracks of six representative B–T encounters normalized to initial B–T contact. OTII T cells are shown in blue, LPS B cells in green, and B cells in red. (D) 3D coordinates of an OTII T cell (blue) and an LPS (green) (Left) or a CpG (red) (Right) mOVA B cell. Bars indicate duration of B–T contact. (E) Distribution of instantaneous velocities of OTII T cells imaged in the B-cell zone when not in contact (gray; arrow at mean = 12.4 ± 0.3 μm/min) and when in contact with an LPS mOVA B cell (green; arrow at mean = 6.4 ± 0.14 μm/min) or a CpG mOVA B cell (red; arrow at mean = 4.0 ± 0.4 μm/min). Data are from four separate experiments. (F and G) Contact maps of OTII T cells (F) and control WT T cells (G) with LPS (green) or CpG (red) mOVA B cells (n = 30 individual cells, three separate experiments). (H) (Left) Duration of contacts of OTII T cells with LPS (mean = 10.3 ± 1.2 min, n = 61 cells; four separate experiments) and CpG mOVA B cells (mean = 3.1 ± 0.74 min, n = 16 cells; four separate experiments). (Right) Duration of contacts of WT T cells with LPS mOVA B cells, (mean = 2.1 ± 0.3 min, n = 38) and with CpG mOVA B cells. Data are from three separate experiments; (mean = 2.2 ± 0.2 min; n = 50). Filled circles represent contacts in which the start and/or finish was not visualized.