Table 1.
Induction of a GSTS1-GLUC reporter construct in WT exposed to different chemical treatments and EC50 ± SEM of the chemicals in WT and sor1
| Chemical | Concentration | Fold induction (±SEM) |
EC50 (μM) |
||
| Light | Dark | WT | sor1 | ||
| Salt or osmotic stress | |||||
| NaCl | 200 mM | 1.8 ± 0.5 | n.d. | n.d. | n.d. |
| Sucrose | 600 mM | 1.5 ± 0.5 | n.d. | n.d. | n.d. |
| Photosynthesis inhibitor | |||||
| DCMU | 0.1 μM | 1.1 ± 0.2 | n.d. | n.d. | n.d. |
| DBMIB | 5 μM | 325 ± 120 | 230 ± 72 | 5.8 ± 0.7 | 5.7 ± 0.3 |
| ROS-generating chemicals | |||||
| H2O2 | 2 mM | 5.9 ± 2.4 | 1.0 ± 0.1 | 2,100 ± 205 | 1,977 ± 294 |
| MV | 0.1 μM | 1.1 ± 0.2 | 1.0 ± 0.1 | 0.056 ± 0.004 | 0.057 ± 0.002 |
| NR | 5 μM | 13.7 ± 2.0 | 1.0 ± 0.1 | 4.1 ± 0.4 | 7.9 ± 0.5* |
| RB | 1 μM | 4.2 ± 1.6 | 0.6 ± 0.2 | 1.3 ± 0.1 | 1.8 ± 0.1* |
| tBOOH | 100 μM | 30.3 ± 9.1 | 12.5 ± 2.1 | 111 ± 4 | 139 ± 4* |
| RES-generating chemicals | |||||
| p-Benzoquinone | 20 μM | 130 ± 8 | 53.2 ± 17.6 | 19.9 ± 0.4 | 30.5 ± 4.0† |
| tBHQ | 30 μM | 27.5 ± 6.8 | 40.3 ± 9.7 | 28.5 ± 1.0 | 30.8 ± 1.6 |
| CDNB | 10 μM | 125 ± 18 | 24.8 ± 6.6 | 8.7 ± 0.4 | 12.6 ± 0.8* |
| 2E-Hexenal | 500 μM | 232 ± 33 | 107 ± 16 | 471 ± 11 | 541 ± 18* |
| Phytohormones | |||||
| OPDA | 200 μM | 23.9 ± 5.6 | n.d. | n.d. | n.d. |
| JA | 200 μM | 1.0 ± 0.1 | n.d. | n.d. | n.d. |
n.d., not determined.
*Significant difference between sor1 and the WT resistance (P < 0.01).
†Significant difference between sor1 and the WT resistance (P < 0.05).