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. 2012 Apr 30;109(20):7799–7804. doi: 10.1073/pnas.1204105109

Fig. 1.

Fig. 1.

Mutational spectra opposite CPDs in UV irradiated mouse (BBMEF) cells treated with Rad18 siRNA. Mutational spectrum was determined for the cII gene in cells exposed to 5 J/m2 of UV light; only the sequence for nucleotide positions 25–288 is shown because the UV-induced mutations are clustered in this region. (A) Mutations resulting from TLS opposite CPDs in UV irradiated cells treated with control (NC) siRNA (above the sequence) or treated with Rad18 siRNA (below the sequence). The (6-4) photoproducts were removed from the genome by expressing a (6-4) photolyase gene and exposing cells to photoreactivating light. The positions of mutational hot spots opposite CPDs (numbers 1–11) are indicated. (B) Mutations in non-UV–irradiated cell line expressing the (6-4) photolyase, and treated with control (NC) siRNA (above the sequence) or treated with Rad18 siRNA (below the sequence).