Fig. 4.

The burst-by-burst recoloring algorithm for data analysis. (A) Molecules diffusing through a laser spot. (B) A sequence of donor (green) and acceptor (red) photons in a burst of duration T. (C) The superposition of the photon sequence, from which the colors have been erased, with a three-state conformational trajectory. (D) Photons emitted by the same conformational state are first grouped together and then recolored using the appropriate binomial distribution (see Methods). Each donor photon in a given state is then assigned a random delay time (arrows). Finally, all photons and delay times from different states are combined.