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. 2012 Apr 30;109(20):7729–7734. doi: 10.1073/pnas.1120251109

Fig. 2.

Fig. 2.

Acetyltransferase activity assay of Mtb Eis and Msm Eis using the recombinant human DUSP/MKP-7(1−153) and DUSP/MKP-7(1−303) proteins as potential substrates. (A) Time-course acetyltransferase activity assay of Mtb Eis and a comparison of Mtb Eis and Msm Eis activities. The wild-type DUSP16/MKP-7(1−153) and DUSP/MKP-7(1−303) were incubated with [14C]-labeled acetyl CoA and Eis for the indicated duration at 37 °C. The reaction products were separated by 15% (wt/vol) SDS/PAGE, and the acetylated protein bands were visualized using a Bioimage analyzer. (B) Acetyltransferase activity of Mtb Eis and Msm Eis toward the wild-type and mutants of DUSP16/MKP-7(1−153). All reactions were carried out at 37 °C for 30 min.