Fig. 4.

Effects of individual cytoskeletal components on whole-cell deformability. (A) Density scatter plots of the initial diameter and deformability of untreated NIH 3T3 cells (control), 3T3 cells treated with latrunculin A, nocodazole, sphingosylphosphorylcholine, and blebbistatin for 2 h. (B) Median and semi-interquartile deviation (SID) statistics of untreated 3T3 cells and 3T3 cells treated with different concentrations of one of four compounds for 2 h (error bars are SID). “Drug-induced substrate release” indicates that cells detached from the substrate into suspension before the 2-h treatment period elapsed.