Fig. 5.

In vivo reporter assay using constructs including the Kiss1 gene locus. (A) Schematic illustration of constructs for the generation of Tg mice. Green boxes indicate the inserted AcGFP sequence. (B and C) Kisspeptin and GFP expression in the AVPV and ARC of OVX and OVX+E2 Tg mice. Photomicrographs show sections of the AVPV and ARC stained by immunohistochemistry for kisspeptin (Left) and GFP (Center). Computer-aided merged images of immunoreactive signals for kisspeptin and GFP are shown on the right. (Scale bars, 100 μm.)