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. 2012 Apr 30;109(20):7765–7769. doi: 10.1073/pnas.1205132109

Fig. 1.

Fig. 1.

The effects of K685R acetylation defective mutant of STAT3 on tumor growth, expression and promoter DNA methylation of tumor suppressor genes. (A) IHC analyses of normal human skin vs. melanoma tissue sections by staining with antibodies against acetylated STAT3 at Lys685. (Scale bars, 100 μm.) (B) Growth curve of A2058 tumors overexpressing either STAT3 wild-type or acetylation mutant (KR) (n = 6); ***P < 0.0001. (C) Real-time RT-PCR analysis of tumor-suppressor gene mRNA expression levels in A2058 tumors (n = 3). (D) Gene-specific promoter methylation analysis by methyl-dependent immunoprecipitation of A2058 tumor cells transfected with either STAT3 WT or KR plasmid expression vectors. Preimmune serum (PIS) was used as nonspecific antibody control. Ordinant shows enrichment over input; shown are means ± SD.