Real-time gene expression conducted on silk microfiber-reinforced scaffolds seeded with hMSCs under differentiating conditions showing fold expression of osteogenic genes: (A) ALP, (B) Collagen 1a1, (C) OP, and (D) BSP. (Scale bar, 200 µm.) Data represents mean ± SD (n = 4), where **P ≤ 0.01 and *P ≤ 0.05.