Fig. 6.

Thymic deletion of P2-specific cells, but not of P7-specific cells, is dependent on antigen presentation by BM-derived APCs. Irradiated WT mice were reconstituted with either WT or CIITA^−/− BM. Four thymi were pooled and the tetramer-binding T cells quantified. Representative FACS plots of CD3⁺CD4⁺ SP and CD3⁺CD8⁺ SP thymocytes are shown. Each data point in the summary graph represents the number of tetramer-positive cells in the four thymi. The dotted line in each graph is the LOD.