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. 2012 Apr 30;109(20):7776–7781. doi: 10.1073/pnas.1111135109

Fig. 3.

Fig. 3.

Involvement of c-Myc in the transcriptional regulation of ST3Gal1/3/4 and FUT3. (A) ChIP assays were performed to examine the binding of c-Myc to the 5′-regulatory regions of ST3Gal1/3/4 and FUT3. (B) Effect of c-Myc shRNA (shMYC) on the transcript level of c-Myc was evaluated by quantitative RT-PCR. (C) Effects of shMYC on the expression levels of ST3Gal1/3/4 and FUT3 were examined by quantitative RT-PCR. (D) Expression levels of sLex/a were examined by flow cytometry. Dotted lines, staining control; bold lines, nontarget shRNA (shNT)-introduced cells; filled histogram, shMYC-introduced cells. (E) Levels of c-Myc and phospho–c-MycSer62/Thr58 were determined by Western blotting. (F) Expression of c-Myc was examined by conventional RT-PCR. (B–D) Statistic analysis was performed in three independent experiments by t test. Error bars, SD; asterisks, P < 0.00001 (B), P < 0.05 (C), and P < 0.0005 (D) compared with the shNT-transfected cells (B) or to the shNT-introduced cells treated with EGF/bFGF (C and D).