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. 2012 May 3;2012:960159. doi: 10.1100/2012/960159

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Copyright © 2012 I. Bravo Portela et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Histochemistry of the sole and side foot (sections are oriented to the bottom for the sole foot and to the right for the side foot, according their natural location) of Haliotis tuberculata. (a) and (b) HID/AB. The sole (a) and side (b) secretory cells in dark containing sulphated acidic glycoconjugates, moreover some subepithelial glands (a, arrows) and side secretory cells (b) stain in blue revealing the presence of carboxylated acidic glycoconjugates. Both glycoconjugates are present in a few secretory cells (b, arrow). Bar: 200 μm (a), 100 μm (b) (c) and (d). PAS. Presence of neutral glycoconjugates in the sole secretory cells and subepithelial gland (c), and the side secretory cells (d) Bar: 200 μm (c), 100 μm (d) (e) UEA I (fucose). The subepithelial glands are strongly reactive to the lectin, and a few secretory cells (arrow) are also positive. Biotinylated lectin, counterstained with hematoxylin. Bar: 100 μm. (f) and (g) AAA (fucose). Some side secretory cells show positivity (f) which increases after desulphation (g) Lectin labeled with digoxigenin. Bar: 100 μm. H. Con A (mannose). The apical portion of the sole epithelial cells and subepithelial gland exhibit reactivity to mannose. Biotinylated lectin, counterstained with hematoxylin. Bar: 50 μm. (i) GNA (mannose). After desulphation GNA binds to some side secretory cells. Lectin labeled with digoxigenin. Bar: 50 μm. (j), and (k) PNA (Galactose beta 1-3 N-acetyl-galactosamine). After desulphation the number of side secretory cells increases (j) and a few sole secretory cells appear (k) Lectin labeled with digoxigenin. Bar: 50 μm. (l) and (m) WGA (N-acetyl-glucosamine). The weak reaction changes after desulphation, increasing intensity and number of sole (l) and side (m) secretory cells. Subepithelial glands appear weakly stained (l). Biotinylated lectin. Bar: 100 μm (l), 50 μm (m) (n), and (o) DBA (N-acetyl-galactosamine). The number of positive side secretory cells increases after desulphation (n) and a high number sole secretory cells appear (o) Bar: 50 μm.