Figure 1.
Levels of Bdnf mRNA and BDNF protein in the cerebral cortex of Bdnfklox/klox mice. A, Representative images of radioactive in situ hybridization of WT (+/+) and Bdnfklox/klox (k/k) coronal brain sections. Arrows denote the visual cortex. The sense probe did not reveal any signals. B, Quantification of radioactive in situ hybridization signal in the visual cortex of +/+ and k/k mice. Error bars indicate SEM. Student's t test, p = 0.253 (4 +/+ mice and 3 k/k mice). a.u., arbitrary unit. C, ELISA analysis of cortical BDNF levels in 5- to 6-week-old +/+ and k/k mice (n = 5 mice per genotype). Error bars indicate SEM. Student's t test, p < 0.001.