Figure 3.

Expression of miR-205 in radio-sensitive cells leads to IR resistance. (A) miR-205 overexpression in CNE-2 cells. CNE-2 cells were transfected with miR-205 expressing vector. Expression of miR-205 was quantitated by q-RTPCR. Expression of miR-205 in the transduced cells was assessed by qRT-PCR with U6 RNA as an internal control. (B) CNE-2 overexpressing miR-205 becomes more IR resistant. Indicated miR-205-overexpressing CNE-2 cells were plated in triplicate and exposed to a range of IR doses (0–6 Gy). The forci-formation was indicated. (C) miR-205-overexpressing CNE-2 cells have induced numbers of forci formation. The numbers of foci-formation were presented as bar graphs. (D) miR-205-overexpressing CNE-2 cells have increased survival fraction. Survival fractions were calculated as described above based on the data from experiments in (C). (E) miR-205-overexpressing CNE-2 cells is resistant to IR-induced cell death. Indicated cells were treated with or without 10 Gy IR and the cells were stained with PI and the percentage of sub-G₁ cells was measured by flow cytometry in three independent experiments. The percentage of sub G₁ cells was presented as bar graphs.