Table 2.
Renal gene expression.
| ANOVA | |||
|---|---|---|---|
| mRNA, strain | P MBL×D | P D | P MBL knockout |
| TGF-β, C57BL/6JBomTac | NS | 2.3-fold (1.7;3.1), P < 0.001 | NS |
| TGF-β, 129S6/SvEvTac | NS | 2.5-fold (1.8;3.6), P < 0.001 | 44% (2;104), P = 0.04 |
| CTGF, C57BL/6JBomTac | NS | 33% (9;64), P < 0.008 | −34% (−19;−46), P < 0.001 |
| CTGF, 129S6/SvEvTac | NS | 2.2-fold (1.5;3.1), P < 0.001 | NS |
| Fibronectin, C57BL/6JBomTac | NS | 2.6-fold (1.9;3.7), P < 0.001 | −30% (−2;−50), P = 0.04 |
| Fibronectin, 129S6/SvEvTac | NS | 3.6-fold (2.4;5.5), P < 0.001 | NS |
| Collagen IVα1, C57BL/6JBomTac | a | P < 0.03b | NSc |
| Collagen IVα1, 129S6/SvEvTac | NS | 2.4-fold (2.1;2.8), P < 0.001 | 16% (0;34), P = 0.04 |
| VEGF-A, C57BL/6JBomTac | NS | −51% (−22;−69), P = 0.004 | NS |
| VEGF-A, 129S6/SvEvTac | NS | −24% (−6;−38), P = 0.02 | NS |
| VEGFR-2, C57BL/6JBomTac | NS | NS | −30% (−14;−43), P = 0.002 |
| VEGFR-2, 129S6/SvEvTac | NS | NS | 41% (12;77), P = 0.005 |
| Nephrin, C57BL/6JBomTac | NS | NS | NS |
| Nephrin, 129S6/SvEvTac | NS | NS | NS |
Expression of mRNA measured by RT-PCR relative to 18S mRNA.
P MBL×D: interaction between diabetes and MBL.
P D: diabetic versus nondiabetic animals.
Values indicate diabetes compared with control (95% confidence interval). P MBL: MBL knockout versus WT animals. Values indicate MBL knockout compared with wild-type (95% confidence interval).
NS: P > 0.05.
a: values could not be fitted to normality and therefore Wilcoxon rank-sum tests were used to test effects of diabetes and MBL.
b: P = 0.025 (WT versus diabetic WT, by Wilcoxon rank-sum tests) and P = 0.028 (MBL-KO versus diabetic MBL-KO, by Wilcoxon rank-sum test).
c: WT versus MBL-KO by Wilcoxon rank-sum test.
One diabetic WT was omitted from analyses for technical reasons.