Table 1.

Summary of the localization patterns observed for TRIM22.

Localization	Pattern	Cell Type	Epitope Tag	Reference
Cytoplasm	Diffuse Diffuse Diffuse Diffuse with speckles/bodies Diffuse Diffuse Diffuse with speckles/bodies	293T COS7 HeLa HeLa HeLa PBMCs U2OS	GFP or V5/His GFP or V5/His Endogenous GFP GFP or V5/His Endogenous GFP	[40] [40] [40] [22] [40] [40] [22]
Cytoplasm & Nucleus	Nucleoplasmic, with nuclear bodies1 Diffuse throughout, or nuclear bodies2 Nucleoplasmic, with nuclear bodies Diffuse, with cytoplasmic bodies3 Nucleoplasmic with NB4 Nucleoplasmic, with nuclear bodies Nucleoplasmic and cytoplasmic Diffuse with speckles5-7	ABC28 HeLa HeLa HeLa MCF7 MCF7 T47D U2OS	Endogenous EGFP Endogenous FLAG EGFP, EYFP, or FLAG Endogenous Endogenous Endogenous	[30] [30] [30] [43] [30] [30] [30] [42]
Nucleus	Aggregates/bodies Aggregates/bodies Diffuse with speckles/bodies Diffuse with speckles/bodies Diffuse with bodies	293 COS7 HepG2 HepG2 MCF7	Myc Myc Endogenous Myc FLAG	[41] [10] [13] [13] [20]

¹Some colocalization with fibrillarin (Nucleoli).

²Pattern changes with cell cycle phase: (G0/G1: nuclear bodies; S-phase: nuclear speckles and cytoplasmic; mitosis: diffuse throughout cell).

³TRIM22 plasmid was coexpressed with Rhesus TRIM5α.

⁴Partial colocalization with Cajal bodies.

⁵Potential colocalization with calnexin (Endoplasmic reticulum).

⁶Localization was primarily cytoplasmic when cells were fixed with paraformaldehyde, or both cytoplasmic and nuclear when fixed with ice-cold methanol.

⁷Partial colocalization with the centrosome.