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Exposure to EDCL induces apoptosis in HepG2 cells. (a) Cells were incubated in the presence or absence of several concentrations of EDCL for 48 h. Hoechst stain showed EDCL-induced chromatin condensation (arrow). Magnification, ×400. (b) Cells were exposed to several concentrations of EDCL for 48 h or (c) exposed to EDCL (180 μg/mL) over time. Apoptosis was measured using PI staining and flow cytometry.
