Table 1.
Data collection and refinement statistics
| IgE-Fc/sFcεRIα | IgE-Fc | |
|---|---|---|
| Data collection | ||
| Space group | P212121 | P21212 |
| Cell dimensions | ||
| a, b, c (Å) | 99.49, 103.34, 110.12 | 130.50, 75.28, 79.14 |
| Resolution (Å) | 3.4 (3.49 – 3.40) | 1.9 (1.97 – 1.90) |
| Rmerge | 0.112 (0.522)a | 0.074 (0.533) |
| I / σI | 8.5 (1.6) | 26.3 (2.9) |
| Completeness (%) | 99.9 (99.9) | 99.9 (100) |
| Redundancy | 5.9 (6.1) | 7.3 (7.4) |
| Refinement | ||
| Resolution (Å) | 37 – 3.4 | 36 – 1.9 |
| No. reflections | 16,204 (1,174) | 62,035 (6,147) |
| Rwork / Rfree | 0.245/0.292 | 0.193/0.234 |
| No. atoms | ||
| Protein | 5,263 | 5,017 |
| Non-protein | 92b | 175c |
| Water | 0 | 533 |
| B-factors (Å2) | ||
| Protein | 77 | 48 |
| Non-protein | 76 b | 77c |
| Water | - | 45 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.006 | 0.005 |
| Bond angles (°) | 1.104 | 1.001 |
Values in parentheses are for highest-resolution shell.
Data for each structure were collected from a single crystal.
a
Rpim precision-indicating Rmerge 16.
b
Carbohydrate.
c
Carbohydrate, SO42−, 2-amino-2-hydroxymethyl-propane-1,3-diol (Tris), polyethylene glycol (PEG).