Skip to main content
. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Mech Ageing Dev. 2012 Feb 8;133(4):176–185. doi: 10.1016/j.mad.2012.01.008

Figure 7. RA treatment increases the reserve capacity of SH-SY5Y cells.

Figure 7

(A) Representative OCR profiles of differentiated (D) and undifferentiated (U) cells upon injection of mitochondrial inhibitor oligomycin (2 μg/ml) followed by injection of rotenone (2 μM) as a function of time. (B) ATP-linked mitochondrial respiration (black bars, obtained from the OCR decrease upon injection of oligomycin) and proton leak (gray bars, obtained from rotenone injection sequentially after oligomycin injection) did not show substantial differences between D and U cells. (C) Representative OCR profiles of D and U cells upon injection of mitochondrial inhibitors oligomycin (2 μg/ml) and mitochondrial membrane potential uncoupler FCCP (0.375 μM). (D) Reserve capacity (obtained from FCCP injection sequentially after oligomycin injection) is significantly higher in D cells than that in U cells. ** p < 0.01 ( n = 3, Student's t-test). Error bars, SD.